Other

Part:BBa_M31005:Design

Designed by: Iny Jhun   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


M13K07 gene 5 refactored


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 328
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any overlaps with other genes in the M13K07 genome were eliminated and restriction enzyme sites were added for easier manipulation of the genes, such as insertion and deletion.


Source

M13K07 genomic sequence.

References