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Other
Part:BBa_M31005:Design
Designed by: Iny Jhun Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07 (2007-02-28)
M13K07 gene 5 refactored
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
Illegal XbaI site found at 328 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
Illegal XbaI site found at 328 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
Illegal XbaI site found at 328 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Any overlaps with other genes in the M13K07 genome were eliminated and restriction enzyme sites were added for easier manipulation of the genes, such as insertion and deletion.
Source
M13K07 genomic sequence.